BIOCHEMICAL AND HEMATOLOGICAL PARAMETERS OF WISTAR RATS AND SWISS MICE IN THE PROFESSOR THOMAS GEORGE

Abstract

Objective: The objective of the present study was to establish reference values for the biochemical and hematological parameters of rodents from the Professor Thomas George animal laboratory of the Pharmaceutical Technology Laboratory, Federal University of Paraíba. Methodology: The parameters evaluated consisted of hematological profile with full blood and platelet counts, as well as serum components including glucose, urea, creatinine, total cholesterol, triglycerides, uric acid, total protein and protein fractions, alkaline phosphatase, transaminases, sodium, potassium, calcium and magnesium. Adult albino Wistar rats and Swiss mice (20 males and 20 females of each species), weighing 150-350 grams and 15-35 grams, respectively, were used in the study. Results: Variations were detected between values obtained in the animals and parameters established in the literature. In rats, leukocyte and eosinophile counts were lower, while in mice, platelet counts, AST, ALT, alkaline phosphatase, globulin, calcium and magnesium levels were higher compared to data published in the literature. Conclusion: In conclusion, the physiological parameters of experimental animals should be well established and made available in view of the variations that may exist as a result of various factors such as sex, lineage and genotype of the rodents that must be taken into consideration in experimental studies. DESCRIPTORS Hematology. Biochemistry. Rats. Mice. Animal Technicians.