ANTIFUNGAL ACTIVITY OF MOUTHWASHES ON CANDIDA SPP.

Abstract

Objective: To verify the in vitro antifungal activity of the mouthwashes Malvatricim® (P1), Colgate Plax® (P2), Oral B®(P3) and Colgate Periogard® (P4) on Candida albicans (ATCC 40277), Candida albicans (ATCC 289065), Candida krusei(ATCC 40147), Candida tropicalis (ATCC 13803) and Candida tropicalis (ATCC 40042). Material and Methods: Fungal suspensions were prepared (106 microrganisms / mL) and seeded on Petri dishes containing Sabouraud Dextrose Agar 4%. It was followed the agar diffusion method by means of the wells technique. Into each well were inserted 50 μL of the products as in their commercial formulation. Nystatin 1:100.000 IU (P5) was used as positive control. The plates were incubated in bacteriological incubator at 37ºC for 48h. Tests were performed in triplicate and compared to positive control. The mean diameter of growth inhibition halos (MDGI)promoted by the substances was calculated and represented the antimicrobial activity. Results: P4 has showed the most potent activity against C. albicans (ATCC-289065), C. krusei(ATCC-40147), C. tropicalis (ATCC-40042), and C. tropicalis(ATCC-13803), with MDGI of 15.0mm; 15.0mm; 18.0mm and 14.3mm, respectively. C. albicans strain (ATCC-40277) was the only one susceptible to P1 (MDGI=25.3mm) and resistant to P5. The mouthwashes P2, P3 and P4 have presented antimicrobial activity against all strains assessed. Conclusion: It is concluded that the products under study, excepting Malvatricim®, have showed inhibitory activity on all strains assayed. Colgate Periogard® has presented the best activity on the strains investigated, followed by Colgate Plax®, Oral B® and Malvatricim®. DESCRIPTORS Dental prophylaxis. Microbiology. Fungi.