ACUTE TOXICITY IN WISTAR RATS TREATED WITH OF Dioclea grandiflora Mart. Ex Benth (Fabaceae) (EEDg) ETHANOLIC EXTRACT

Abstract

Introduction: Dioclea grandiflora, known as Mucunã de caroço, acts on the central nervous system, and against prostate disease, and kidney stones. Objective: Conduct nonclinical acute toxicology study in rats, based on Normative Instruction # 4, June 18th 2014 of the National Health Surveillance Agency (ANVISA). Methodology: Wistar rats were used, both sexes, a dose of 2000 mg/Kg ethanol extract of Dioclea grandiflora was administered orally to the treated group with a control group (vehicle). After the administration, behavioral parameters were observed for 30, 60, 90, 120, 180, and 240 minutes after feed and water intake, along with hematological and biochemical parameters. The number of survivors was recorded to determine the LD50. Results: There was a statistically significant increase in water consumption (Control: 160.4 ± 5.85; Treated: 201.3 ± 8.55), and feed for the female mice (control: 95.98 ± 3.02; Treated: 113.1 ± 2.42), and statistically significant increases in water consumption (control: 236.7 ± 6.43; Treated: 267.5 ± 8.72) and feed the males (control: 152.4 ± 2.51; Treated: 177.6 ± 4.15). Statistically significant albumin increase in rats (Control: 3.2 ± 0.08; Treated: 3.6 ± 0.08), for female alkaline phosphatase we found a statistically significant reduction (control: 198.5 ± 18.81; Treated: 99.97 ± 16.02), total protein (control: 7.85 ± 0.09; Treated: 6.85 ± 0.24) and globulin (Control: 4.28 ± 0.14; Treated: 3.27 ± 0.27). Also, in females the number of red blood cells decreased significantly (control: 10.18 ± 0.28; Treated: 9.62 ± 0.18). Conclusion: According to the results, the LD50 value was higher than the tested dose. However long-term toxicology studies are needed to prove the extract’s safety. Descriptors Central nervous system. Acute toxicity. Wistar rats.