EFFECT OF AGING ON THE IN VITRO EXPANSION OF BONE MARROW HEMATOPOIETIC CELLS

Authors

  • Alessandra Marinho Miranda
  • Fernanda Ginani
  • Carlos Augusto Galvão Barboza

Abstract

Objective: This study aimed to evaluate the influence of aging on the proliferative capacity of hematopoietic cells obtained from mice bone marrow. Material and Methods: two groups of animals were compared: young (30 days old) and senile (18 months old). Bone marrow extracts were collected from the medullary cavity of the tibia and femur of the animals and the hematopoietic cells were cultured and counted in intervals of 24, 48 and 72 hours. Results: The results demonstrated that in young animals the growth curve of the cells showed a proliferative trend, but in senile animals it was observed a decreasing curve, with an intense fall in the 72 hours interval. Conclusion: it’s concluded that the advanced age of the animal exerts a negative influence in the in vitro yield of bone marrow hematopoietic cells, which may represent an important factor to be considered in protocols that use those cells for therapy of diseases affecting the hematopoietic system. Descriptors: Bone marrow. Hematopoiesis. Aging.

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Published

2011-02-25

How to Cite

Miranda, A. M., Ginani, F., & Barboza, C. A. G. (2011). EFFECT OF AGING ON THE IN VITRO EXPANSION OF BONE MARROW HEMATOPOIETIC CELLS. Revista Brasileira De Ciências Da Saúde, 14(3). Retrieved from https://periodicos.ufpb.br/ojs2/index.php/rbcs/article/view/8223

Issue

Vol. 14 No. 3 (2010)

Section

Research

License

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