Crescimento de Chlorella Vulgaris (chlorophyta unicelular) na presença de compostos fenólicos
Keywords:
Chlorella vulgaris, Extractos de Rosmarinus officinalis, Compostos fenólicos, Estimulação/inibição do crescimento em microalgasAbstract
Neste trabalho avaliou-se o efeito de diferentes tipos de extractos da planta de rosmaninho (Rosmarinus officinalis) ricos em compostos fenólicos, no crescimento e na fisiologia da microalga Chlorella vulgaris. Os extractos foram obtidos por infusão aquosa a quente e a frio, esta ultima com dois períodos de extração (5 e 21 dias). Os ensaios foram desenvolvidos em culturas batch com extratos à concentração de 10%, 25% e 30% (v/v). Os resultados mostraram para os extractos a frio que o crescimento da microalga foi afetado apenas com extratos de 21 dias, levando ao aumento da taxa específica de crescimento (μ) e da densidade celular, bem como da concentração de clorofila a (Chl a) e do teor em proteinas, numa relação direta com a concentração de extrato testada. A exposição aos extractos a quente apesar de levar ao aumento de µ e da densidade celular final de C. vulgaris, concomitantemente induziu alguma toxicidade a nível fisiológico, visível pela diminuição dos teores de Chl a e proteínas. Os compostos fenólicos identificados por HPLC, nestes dois tipos de extratos, mostraram diferenças qualitativas e quantitativas. O extrato a frio apresentou essencialmente ácido rosmarínico e quercetina, enquanto que no extrato a quente foram quantificados sete compostos distintos, sendo os mais abundantes os ácidos rosmarínico e gálico. Considerando o perfil de compostos fenólicos encontrado, é possível concluir que o grau de inibição/estimulo do crescimento de C. vulgaris está relacionado com a concentração e composição em compostos fenólicos dos extractos estudados.Downloads
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